Super Mario Advance 5: Super Mario Land

Changes from Super Mario Land

 * The game is now in color and has 16-bit graphics.
 * Superball Mario is now differently colored from Super Mario.
 * The 1-Up Heart was replaced with a 1-Up Mushroom.
 * Mario's design is now derivative from his design in the 16-bit iterations of Super Mario Bros. 2/Super Mario USA.
 * The game now supports saving (via three save slots).
 * After getting a Game Over, you simply restart from the world you left off on.
 * You can no longer continue after getting 100,000 points due to this.
 * The enhanced Mario Bros. arcade game from the past four installments and Superstar Saga was added in.
 * The levels now have "MARIO START" before they begin, similar to Super Mario World and the 16-bit versions of Super Mario Bros. 3.
 * Mario now starts at 3 lives and dies instead of reaching the zero counter.
 * The flies with arrows are now referred to as "Cupid Troopas" and given a massive redesign.
 * Luigi is now a playable character, with different physics.
 * Voice clips from Charles Martinet were added in, similar to Super Mario Advance, Super Mario Advance 2 and Super Mario Advance 4.
 * Princess Daisy is given her modern design, and now has voice lines provided by Deanna Mustard.
 * In World 2, you can now swim in the water for about three seconds instead of instantly dying.
 * The Star music is now the same as it was in Super Mario Advance 4 instead of playing the Can-Can.
 * The bonus room theme is now a remix of the Super Mario All-Stars bonus theme.
 * The submarine no longer shrinks down or grows with Mario, and is full size the whole time.
 * The boulders in World 3-1 now have sound effects.
 * The cave flies in World 3-2 are replaced with the Super Mario 64 design of Thwomps, which is merely an aesthetic difference.
 * The spiders in World 3-2 were replaced by Scuttlebugs from Super Mario 64.
 * The waterfalls in World 3-2 were altered to reduce risk of seizure.
 * World 3-3 now has the Overworld theme rather than the Underground theme.
 * Hiyoihoi now is voiced by Charles Martinet rather than using the default boss sounds.
 * The Pinopies have all been replaced by Koopa Troopas on pogo sticks.
 * The giant flowers in World 4-2 have been replaced by giant Nipper Plants.
 * The sky plane in the final level now has eyes.
 * Tatanga now has full voice lines, provided by Charles Martinet, using the same voice he used for Wart in Super Mario Advance.
 * The credits now show the names in full instead of merely the first initial, last name.
 * The ending screen now unlocks a Hard mode and the ability to play as Princess Daisy.
 * The game is now compatible with e-Reader cards in Japan, and lock-on carts elsewhere.
 * A link cable can be used to allow two-player play as Mario and Luigi.

List of e-Reader/lock-on content
The game is well-known for the large amount of crossover characters who could replace Mario and Luigi depending on the card/lock-on cart used.