The logo more resembles that of the American Mario 3 logo
All sounds are PAL pitched (Eruowood version only)
The game now has Pepsi billboards due to Nintendo's Pepsi sponsorship in Eruowood at the time (Eruowood version only)
Grabbing a fire flower as small Mario now brings you to Fire Mario
After beating the game twice, all Fire Flowers are replaced with Raccoon Suits (non-underwater levels) or Frog Suits (underwater levels)
After beating the game 5 times without dying or warp zones, all Fire Flowers are replaced with Tanooki Suits
Buzzy Beetles no longer replace Goombas upon beating the game
The ending was made to resemble Mario 3's ending
The minus world glitch is fixed, but due to the game being made with PAL in mind, Minus World is possible to access on NTSC CRT TVs
At the end of the game, there is text saying "Super Mario Bros: Super Edition, Coming Soon!", this would be a teaser for Super Mario Bros: Super Edition a port of Super Mario Bros: The Lost Levels.